PROPERTIES OF BACTERIOLYSANTS 



479 



had been attiickcd in varying degrees l)y filtrates were centrifuged 

 and the sediment stained. The amount of cellular debris was 

 variable but the number of morphologically typical dysentery 



TABLE 2 



Comparison of bacleriobjlic aclivily of bacterial ysanls with the bacterial counts of the 



culture before and after the action of bacleriobjsants 



5 CC. OP EACH OF THE FOLLOWINQ 

 BACTEKIOLTBANTS WERE ADDED TO 2 0C. 



OP AN EIGHT Horn PEPTONE WATER 

 CULTURE OF FLEXNER Y, AND THEN IN- 

 CUBATED TOOETHEH AT 37°C. FOR 

 TWENTT-rOUR HOURS 



F24(3)A 



F49(2) 



F43(l) 



F44 



(Control) 1 per cent sterile 

 peptone water 



NUUBKR or 



OKOANI8\!a PKR 



CUDICCKXTtMtrnCB 



IN THK EIGHT 



HOUR PEITONK 



WATKn CULTURE 



OF KLi;XNi:U T, 



DKFOUK 



BACTERIOLYBA NT 



WAS ADbKU 



100,000.000 + 

 100,000,000 -h 

 100,000,000 4- 

 100,000,000 -h 



100, 000, 000 4- 



DKGREK OF LT8TB 



AITERCULTURK AND 



DACTERIOr.TSANT 



WEHK INCUBATF:n 



TOQKTHKU AtST^C. 



FOR TWKNTT-FOUR 



HOURS 



+ 



+ 



+ 

 



NUMBER OP 

 riADLE OUOAN18M8 

 PKR CUBIC CENTI- 

 METER AFTER 

 CULTURE AND 

 DACTERIOLYSANT 

 WERE INCUBATED 

 TOGETHER AT 37''C. 

 FOR TWENTY- 

 FOUR HOURS 



264 



44 



2 



130 

 100, 000, 000 + 



TABLE 3 

 Comparison of the degree of bacteriolysis with appearance of the stained sediment 



. 6 CO. OF EACH OF TH E FOLLOWING 

 BACTERIOLYSANTS WERE ADDED TO 2 CC 



OP AN EIGHT HOUR PEPTONE WATER 

 CULTURE OF FLEXNER Y, AND THEN IN- 

 CUBATED TOGETHER AT 37°C. FOR 

 BEVENTY-TWO HOURS 



DEGREE OF LYSIS 

 AFTER THE 

 CULTURE AND 

 BACTERIOLYSANT 

 WERE INCUBATED 

 TOGETHER AT 

 ST^C. FOR SEVENTY- 

 TWO HOURS 



F 24 (3) A 



F49 (2) 



F34 (1) 



F43 (1) 



F31 



F45 



F4S 



F44 



(Control) 1 per cent sterile 

 peptone water 



+++ 

 ++ 

 ++ 

 + 

 + 

 + 

 + 







APPEARANCE OP THE STAINED SEDIMENT 

 (gram's STAIN) AFTER THE CULTURE 



AND BACTERIOLYSANT HAD BEEN 



INCUBATED TOGETHER AT 37°C. FOR 



SEVENTY-TWO HOURS AND 



THEN CENTRIFUGED 



Amorphorus 

 cellular debris 



± 



+ + + 



+ + 



± 



+ + 



+ + + 



+ + + 



+ 







Morphologically 



typical dysentery 



bacilli 



± 

 ± 

 ± 



+ + 



+ + 



+ + + 



+ + + 



bacilli was inversely proportional to the amount of lysis that 

 had occurred (table 3). 



