PKOPERTIES OF BACTERIOLYSANTS 



489 



RESULTS OF INOCULATIONS OF BACTERIOLYTIC FILTRATES INTO 



ANIMALS 



Four doses of 5 to 15 cc. of a bacteriolytic filtrate at intervals 

 of six to seventy-five daj's were injected intravenously and sub- 

 eutaneously into a rabbit. The animal showed no ill effects. 

 Its serum after the fourth injection agglutinated three strains 

 of Flexner bacilU in a dilution of 1:250 and would not agglu- 

 tinate Bact. coU at that dilution. Precipitin tests with this 

 rabbit's serum and a bacteriolytic filtrate were positive in a 

 dilution of 1:2, and negative at 1:20. 



SUMMARY 



The filtrates of the stools of infants suffering from bacillary 

 dysenteiy (Flexner), acute intestinal indigestion, otitis media 

 and the need for regulation of feeding were bacteriolytic for 

 one or more of twenty-seven strains of Flexner Shiga and ty- 

 phoid bacilU. The technique by which these filtrates were 

 obtained and tested, is described. D'Herelle's phenomenon is 

 apparently non-specific and a bacteriolysant from a patient's 

 stool may not be as active against the organism causing that 

 patient's disease as against other strains. It does not necessarily 

 play a part in the immunity or defense-mechanism of the body for 



