NATURE OF BACTERIOLYSANTS 



493 



This culture was then incubated at 37°C. for eighteen hours and 

 150 cc. of it was filtered. This filtrate was strongly bacteriolytic 

 for normal Flexner bacilli. The remaining 350 cc. was then 

 centrifuged at high speed and the supernatant fluid filtered. 

 This filtrate was also strongly bacteriolytic. The sediment of 



TABLK 10 



Bacteriolytic activity of filtrates of peptone water cultures of a "sensitive" strain of 



Bad. dyscnteriae {Flexner Y, Hiss and Russell) 



FILTRATE OR SUSPENSION USED 



Filtrate of eighteen-hour peptone water cul- 

 ture of the eiphth generation of a "sensitive" 

 strain of Flexner bacilli 



Filtrate of supernatant fluid of an eighteen- 

 hour peptone water culture of the eighth 

 generation of a "sensitive" strain of Flexner 

 bacilli after the organisms had been cen- 

 trifuged out 



Saline suspension of the organisms of an eigh- 

 teen-hour culture of the eighth generation of 

 a "sensitive" strain of Flexner bacilli, after 

 they had been centrifuged out and washed 

 twice with saline. This suspension had stood 

 at room temperature for 35 days to allow the 

 organisms to disintegrate 



Filtrate of ninety-six-hour peptone water cul- 

 ture of the thirty-fourth generation of a 

 "sensitive" strain of Flexner bacilli 



Sterile 1 per cent peptone water (control) 



DACTKniOLYTIC 



ACTIVITT OF 



FILTRATE AGAINST 



CrLTTRES OF 



NORMAL FLEXNER 



BACILLI 



PRESENCE OF 

 "sensitive" COLO- 

 NIES IN AGAR 

 SUBCL-LTrHES OF 

 DYSENTERY 

 DACILLI AFTER 

 THEY HAD BEEN 

 INCUBATED WITH 

 THE FILTRATE FOR 

 TWENTY-FOUR 

 HOURS AT SyC. 



+ + 



+ + 



+ + 



+ + 



organisms was washed twice with 15 cc. of 0.9 per cent saline and 

 then suspended in 5 cc. of saline. This suspension was left at 

 room temperature for thirty-five days. Subcultures were then 

 sterile. This suspension was also bacteriolytic. In other words, 

 the lytic principle is both extracellular and intracellular for the 

 fluid medium in which a "sensitive" strain was grown and also 



