502 



WILBTJRT C. DAVISON 



trypsin (Fairchild and Foster) were made in normal saline and 

 20 per cent alcohol. These were proven sterile by culture. As 



TABLE 16 

 Absence of bacteriolytic activity in trypsin 



TABLE 16 



Absence of gelatin liquefying enzyme in bacteriolysants and cultures of "sensitive" 



and "normal" strains of Bad. dysenteriae {Flexner Y) {Hiss and Rxissell) 



BACTERIOLYSANT, CULTURE OR SOLUTION USED 



F 100 (actively bacteriolytic filtrate) 



"Moth eaten" colonies of "sensitive" strain of Flexner Y 



bacilli 

 Regular colonies of normal strain of Flexner Y bacilli 

 5 per cent alcoholic solution of trypsin 

 5 per cent saline solution of trj'psin 



LIQUIFICATION OF 

 GELATIN AT 

 ROOM TEMPERA- 

 TURE 



(34 days) 

 (48 days) 



(48 days) 

 + (24 hours) 

 + (24 hours) 



shown in table 15, neither these solutions, nor filtrates of dys- 

 entery cultures which had been incubated with these solutions 

 were bacteriolytic. That the trypsin used was active and could 

 liquefy gelatin is shown in table 16. 



