METHODS OF PURE CULTURE STUDY 



REPORT OF COMMITTEE ON BACTERIOLOGICAL TECHNIC 



H. J. Conn, chairman, K. N. Atkins, II. J. Brown, F. Eberson, G. E. Harmon, 

 G. J. HucKER, F. W. Tanner, and S. A. Waksman 



Received for publication June 9, 1922 



Several years ago the committee on the descriptive chart was 

 asked by the Society to prepare a manual of methods to be used 

 in connection with the chart, in the pure culture study of bac- 

 teria. As at that time the methods were felt to be very crude, 

 and as considerable work was then in progress on them, it seemed 

 that the best thing that could be done was merely to draw up a 

 short report on the subject, which was printed in the Journal 

 (Committee on Descriptive Chart, 1917) and was distributed 

 in separate form with the charts that were ordered. A limited 

 edition of these separates was printed with the expectation of 

 getting out a revision shortly. Such a revision was prepared 

 a few years later (1920) and at the present time there is need 

 of a second revision. 



This work has now been put in the hands of the more recently 

 organized committee on bacteriological technic and this com- 

 mittee has drawn up at the present time a third edition of the 

 report on methods of pure culture study which is to replace 

 the second edition now almost out of print. So much material 

 has been collected, however, in the last few years and the methods 

 are now so much more satisfactory than they were when the 

 first edition was prepared that it seems advisable to the com- 

 mittee to make this third edition a separately pubUshed manual 

 of methods, according to the instructions given to the earlier 

 committee. 



This manual, as now prepared, contains to a large extent 



methods that have been printed in the earlier editions of the 



report; but for certain procedures, new methods have been 



described. 



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