BACTERIAL AUTOLYSIS 567 



+ 1.5 to +2.5 (Fuller's scale), no autolytic changes could be 

 observed. The other curves on these graphs present a general 

 similarity, except Sorensen 3 and 7, in spite of the fact that 

 they rejjresent material grown on two different media, and for 

 varying lengths of time, and that some of the suspensions were 

 made in distilled water and others in physiological saline solution. 



-i p:.-^ f 





^ 



-.^=^-0- 



10 IS ZO 2S JO 3S 4a 



time: ^v oats 



Chart 10. Pneumococctjs 



Additional experiments with the pneumococcus and meningococcus 



The following experiments were made possible by the generous 

 cooperation of the Lederle Antitoxin Laboratories at Pearl 

 River, New York. It was only by having use of the faciUties 

 of this laboratory and special assistance of a technical nature 

 that the amounts of bacterial growth necessary for carrying out 

 this work could be obtained. 



The pneumococcus was grown in fresh extract broth, con- 

 taining 0.1 per cent glucose, which had long been used for the 



