BACILLUS HEMOGLOBINOPHILUS CANIS 



581 



Pathogenicity. One cuhir centimeter of a twenty-four-hour 

 culture in blood broth did not kill a white mouse when given 

 intraperitoneally; two cubic centimeters given intraperitoneally 

 did not kill a small pn-iinea pig; one cubic centimeter intravenously 

 did not kill a small rabbit. 



From table 1 it can be seen that H. canis can be differentiated 

 from H. pertussis by indole production, nitrate reduction and 

 sugar fermentations; from H. influenzae by growth accessory 

 factors and mannitol fermentation. 



TABLE 1 



Most important differential cultural characteristics of H. pertussis, H. influenzae 



and H. canis 



BACTERI GM 



H. per- 

 tussis 



H. influ- 

 enzae 



H. canis 



ACCESSORY 



OROvrrn factors 



Old cultures require 

 neither autoclave la- 

 bile nor autoclave 

 stable factor 



Autoclave labile and 

 autoclave stable fac- 

 tors required 



Only autoclave stable 

 factor required 



INDOLE 

 PRODCCriON 



Never pro- 

 duced 



May or may 

 not be 

 produced 



Always 

 produced 



NITRATE 

 REDUCTION 



Never re- 

 duced 



Always 

 reduced 



Always 

 reduced 



SUGAR 

 FERMENTATIONS 



No sugar fermented 



Different sugars fer- 

 mented ; never 

 mannitol 



Different sugars 

 fermented, in- 

 cluding mannitol 



CONCLUSIONS 



B. hemoglobinophilus canis of Friedberger has been further 

 described and differentiated from H. pertussis and H. influenzae 

 by cultural methods. According to the new classification this 

 bacillus should be called Hemophilus canis. 



REFERENCES 



Friedberger, E. 1903 Centralbl. f. Bakteriol., I; Orig., 33, 401. 



Odair,\ 1912 Centralbl. f. Bakeriol., Orig., 61, 289. 



Rivers, T. M. 1919 Bull. Johns Hopkins Hosp., 30, 129. 



Rivers, T.M., AND KoHN.L. A. 1921 Jour. Exper.Med., 34, 477. 



Rivers, T. M., and Poole, A. K. 1921 Bull. Johns Hopkins Hosp., 32, 202. 



