UTILIZATION OF PROTEID AND NON-PROTEID NITROGEN 17 



immediately after inoculation and after definite periods of incu- 

 bation at 30°C. The results are given in the accompanying 

 Tables I and II. 



With very few exceptions, the results show that there was 

 httle multiplication of the bacteria with which the medium was 

 inoculated. An increase of 100 per cent, or even 1000 per cent, 



TABLE I 



Enumeration of bacteria in inoculated test medium containing heated {coagulated) 

 egg albumin. Counts per cubic centimeter of medium* 



ORGANISMS 



Prot. vulgaris I . . . . 



Prot. vulgaris II 



Prot. vulgaris III. . . 

 Prot. vulgaris IV . . . 

 Prot. vulgaris V . . . . 



Prot. mirabilis 



B. subtilis 



B. prodigiosus 



Staph, aureus II. . . . 



Staph, aureus II 



Staph, aureus III. . . 

 Staph, aureus IV . . . 

 Staph, aureus V . . . . 

 Staph, aureus VI . . . 



B. coli I 



B. coli II 



B. coli III 



B. typhi I 



B. typhi II 



4 WEEKS 

 AFTER 



INOCULA- 

 TION 



1,775 



1,180 



XX 



XX 



406 



53 



675 



350 



7,200 



162 



Note: XX indicates too many colonies on the agar plates to count. 

 * Dilutions of 1 : 10,000 were employed in these tests. 



in the numbers of organisms would not be unexpected even in 

 what may be termed a nitrogen-free medium which is constantly 

 exposed to the atmosphere. In the tests with B. prodigiosus, B. 

 subtilis and one of the Proteus vulgaris strains the numbers of 

 colonies on the agar plates became too numerous to count. 

 Furthermore, there were visible indications that the protein was 

 undergoing disintegration. These results are, therefore, in strik- 



