212 BERTHA VAN H. ANTHONY AND C. V. EKKOTU 



the material (meat and watery infusion) was divided into three 

 lots: 



B* 30 was kept at the boiling point 30 minutes. 

 B 60 was kept at the boiling point 60 minutes. 

 B 120 was kept at the boiling point 120 minutes. 

 (Volumes being made up by addition of tap water.) 



After being boiled, each lot was strained through cheese- 

 cloth, then filtered through paper (S. & S. "Falten" filter) and 

 cotton (first moistened with cold water to hold back the fatty 

 substances.) 



Each lot was titrated^ and then divided again into two parts 

 and one series (B 30, B 60, B 120) was run in the autoclave at 

 least six successive times, without the addition of soda. 



The second series (B 30 C, B 60 C, B 120 C) was corrected 

 with normal sodium hydroxid to 1 per cent acidity ( + 1) and 

 then run in the autoclave with the other set and under the 

 same conditions. 



In all the tests made the autoclaving was done at a pressure 

 between 15 and 17 pounds as indicated by a Bristol recording 

 pressure gauge. The heating was carried on up to a total of 

 eight hours and titrations performed at one-half hour intervals 

 for the first four half -hours; then at one hour, two hour and 

 three hour intervals. 



After each autoclaving the six samples were titrated and the 

 corrected series (B 30 C, B 60 C, etc.) was adjusted again when 

 necessary to plus one (+ 1). 



Method of titration. Freshly boiled and cooled distilled water 

 was used for all titrations. A 5 cc. sample of meat infusion was 

 drawn off by means of a 5 cc. pipette and added to 45 cc. of dis- 

 tilled water in a casserole to which 1 cc. of a 1 per cent^ solution 



* Preliminary titrations on samples before boiling had been labelled "A." 

 As these had no significance they are omitted in this article. 



'See: "Method of Titration." 



" These are also variations from the standard method of using 0.5 per cent 

 solution of phenolphthalein and twentieth normal sodium hydroxid solution. 

 They are, however, in accordance with the methods in use for years in this labora- 

 tory and to preserve uniformity they were adhered to. 



