TITRATION AND ADJUSTMENT OF CULTURE MEDIA 



219 



room temperature titration are in general nearer to the de- 

 sired reaction of plus one (+1), especially in the first two half- 

 hours — the periods of time of greatest practical interest. 



In chart 7 are shown not only the uncorrected portions of 

 Bi 60 (y and 7') and Bi 120 (7 and 7') (duphcate material from 



CHART 7. 



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? ^- 



k ' 



Q 

 



first lot of veal), but also the efi'ect on the corrected portions of 

 the addition of too much normal sodium hydroxid. By mis- 

 take after the first half hour in the autoclave and the subse- 

 quent titration, there was added to Bi 60 C /3' approximately 

 five times the amount of normal sodium hydroxid necessary 

 to correct it to plus one, and to Bi 120 C jS' also about five times 



