220 



BERTHA VAN H. ANTHONY AND C. V. EKROTH 



the right amount. These amounts were in accordance with 

 the boiling titration figures in both cases. The quantities needed 

 for correction were, in round numbers, twice as much for the 

 latter as for the former. The relation of the quantities of alkali 

 added may, therefore, be expressed by the numerical relation 

 of ten to five. The portions of meat infusion (without further 

 additions of soda) were then run as usual in the autoclave with 

 the uncorrected portions and titrated at the same intervals. 



Curiosity led us to continue these tests rather than discard 

 them. In consequence, an interesting fact was brought out. 

 To our surprise both over-corrected portions recovered the 

 acidity of plus one (and more) but at different intervals of time. 

 Bi 60 C/3' gained plus one at the end of the fifth hour while 

 Bi 120 /3' reached plus one at about the seventh hour. 



In all the tests the curves show a distinct and steady rise in 

 acidity. This rise is due to hydrolysis caused by heat and in- 

 creases continually as more heat^" is applied. Further, it is 

 plainly evident that a preliminary heating to the boiling point 

 for at least forty-five minutes as advocated by Eyre (1915) 

 does not produce a stable reaction uninfluenced by further heating. 



TABLE I 



*R. T. = room temperature titration. 

 *B. T. = boiling temperature titration. 



In the above tests we have gone outside the limits of inter- 

 est from the practical standpoint. We were led to this, however, 

 in an attempt to locate the point of complete hydrolysis or maxi- 

 mum acidity of meat infusions. This goal was not reached, 



10 Three of the uncorrected portions, B2 30, B2 60, B2 120 were run an additional 

 six hours, making fourteen hours all told in the autoclave at 15 pounds pressure. 

 The results are shown in last colimins of Table I. 



