238 



WM. L. OWEN 



tions of aniline dyes, and are Gram positive. The rods are 

 non-motile, non-flagellated, and do not form endospores. 



Physiological characteristics. This species does not liquefy 

 gelatine at all. Milk is rendered slightly acid, and gas is devel- 

 oped after an incubation of 24 hours at 35°C. The consistency 

 of the milk is unchanged in three days. Nitrates are not re- 

 duced. A fairly good growth occurred in a Novy jar from 

 which all of the air was exhausted by means of a vacuum pump, 

 and with the bottom covered with pyrogallic acid solution. The 

 species is therefore a facultative anaerobe. 



The following sugars are fermented by this species: 



Cultural characteristics. On plain agar the colonies are small, 

 rounded, but slightly raised, with entire edges. The surface 

 is smooth and moist, with an amorphous interior structure. 

 On glycerin agar the colonies are round greyish white, with a 

 more glistening surface, but otherwise similar to the colonies 

 on the plain agar. The sub-surface colonies are surrounded 

 by gas bubbles resulting from the fermentation of the glycerin. 



On agar streaks the growth is exceedingly rapid. Inoculated 

 tubes show a marked growth along the line of the needle after 

 six hours' incubation at 35°C. 



On potato the organism forms a dirty white echinulate growth, 

 slightly raised, with a glistening lustre. The growth is of a 

 butyrous consistency. In bouillon the growth is very rapid, 

 and the solution quickly becomes cloudy throughout. No film 

 is produced. In bouillon containing 2 per cent of sucrose a 

 vigorous fermentation follows inoculation with this species. 

 The medium is rendered acid, and the acidity on the third day 

 gives an acidifying coefficient of 3.8. 



In its morphological and physiological characteristics the 

 species in question strikingly resembles other species previously 



