A NEW SPECIES OF ALCOHOL FORMING BACTERIA 



239 



isolated. In the table given below and in that on page 240 will 

 be found the most prominent distinguishing features. 



From the foregoing table of characteristics of the various 

 species of alcohol forming bacteria, and from that on page 240, 

 it will be noted that the species isolated from sugar cane, differs 

 from the others in the following essential points. 



FROM 

 BAC. FITZIANUS 



In smaller size 

 and absence of 

 spores 



FROM 

 BAC. ETHACETICU8 



Not liquefying 

 gelatine ; ab 

 sence of mo 

 tility 



FROM 



BAC. ETHACETO 



8UCCINICU8 



Growth on agar 

 and gelatine; 

 absence of 

 motility 



FROM HENNBBBRO BACILLI 



Non - liquefac 

 tion of gela 

 tine; absence 

 of motility 



Absence of mo- 

 tility; strong 

 fermentation 

 of sucrose, 

 glucose, and 

 levulose 



The observed differences in the characteristics of this species, 

 seem sufficient to constitute it as a new species. Owing to its 

 prevalence in borer infested sugar cane, the name Bacillus sac- 

 charalis seems appropriate. , , -u- 



In order to determine the amount of alcohol formed by this 

 species in the fermentation of glycerin, a solution was pre- 

 pared according to the formula of Frankland and Fox (1889), 



which is as follows: ^^^^ 



60 



Glycerm 2 



Peptone • 2q 



Calcium carbonate (precipitated) 



