AEKOBIC SPOKE-BEAKING NON-PATHOGENIC BACTERIA 297 



Saccharose. Turbidity in open bulb and usually no scum. 

 Turbidity in closed arm. Reaction acid. 



Lactose. Turbidity in open bulb. No scum. Arm clear. 

 Reaction alkaline. 



Thermal death point. Spores survive one hour's heating in 

 Arnold sterilizer and autoclaving at 19 pounds pressure. Killed 

 by 20 pounds pressure. 



Bacillus globigii Migula. 



This organism was originally described by Globig (1888) 

 as Bacillus mesentericus-ruber. A culture was obtained from 

 Krai's Laboratory in Vienna which has the same cultural re- 

 actions as those given by Globig. 



Morphology. Homogeneous bacilU measiuing 0.5 by 2 to 3 

 microns in 24 hours agar cultures. On glucose agar the organisms 

 are longer and slightly thicker often growing out into long 

 chains but short forms are also frequently seen (Figure 14). 



Spore formation. Spores are formed very sparsely and at 

 a late period in the present culture. They are usually seen only 

 in 16 to 18 days giowth and are then characteristic mesentericus 

 spores. 



Motility. Actively motile in 24 hour cultures. 



Staining properties. Gram-negative. 



Agar slant. Thin, spreading, glassy, soft, yellowish-white 

 growth along line of inoculation. 



Agar stab. Slight uniform growth along Hue of puUcture with 

 spreading amoeboid surface growth. 



Agar colonies. Dense, soft, white amoeboid colonies similar 

 to those of Bacillus mesentericus. 



Litmus glucose agar slant. Thick, narrow, white growth 

 along line of inoculation. The medium shows an acid reaction. 



Litmus glucose agar colonies. Thick, round, raised, soft 

 colonies later turning yellowish and rarely pinkish. Medium 

 acidified. 



Gelatin stab. Growth along hne of inoculation and shght 

 surface growth with liquefaction of the gelatin. 



