AEROBIC SPORE-BEARING NON-PATHOGENIC BACTERIA 309 



bits of protoplasm at the ends and then they resembled the 

 mesentericus spores slightly. 



Agar slant. Thin, soft spreading, whitish growth later be- 

 coming yellow. Easily scraped off the agar. 



Agar stab. Faint growth along line of inoculation and spread- 

 ing on the surface, thick and whitish in old cultures. 



Agar colonies. Surface colonies round or bizarre, thick, 

 white. Under low power granular with dense central nuclei. 

 Edges entire. Deep colonies punctiform. Under low power 

 irrregular, with entire edges. 



Litmus glucose agar. Thick, soft, whitish growth along line of 

 inoculation becoming yellowish and irregularly heaped up. 

 Medium quite markedly acidified. 



Litmus glucose agar colonies. Surface colonies round or ir- 

 regular, thick, whitish. Under low power granular and fre- 

 quently show dense central nuclei with thin peripheries showing 

 regular edges. Deep colonies punctiform. Under low power 

 irregular with irregular edges. Reaction of medium acid. 



Gelatin stab. Faint growth along line of inoculation with 

 surface hquefaction and scum production. 



Gelatin colonies. Thin, circular colonies, under low power 

 granular. 



Broth. Turbidity at first, then the medium clears and a dense 

 surface growth appears which shows many clear, globular masses 

 like globules of fat floating on the surface. 



Peptone. Turbidity with a faint fragile jseum. 



Potato. Thin, spreading, moist, yellow growth. 



Litmus milk. Slow decolorization of the Utmus with peptoni- 

 zation and the production of an amber-colored fluid. 



Blood serum. Thin, whitish growth. No Hquefaction. May 

 appear finely wrinkled. 



Fermentation tubes. Glucose. Turbidity in bowl with surface 

 growth and flocculent precipitate. Arm clear. Reaction acid 

 at the end of 2 to 3 days. 



Saccharose. Turbidity in bowl with sHght surface growth. 

 Arm clear. Acidity at the end of 2 to 3 days. 



Lactose. Turbidity in bowl with very shght surface growth. 

 Arm clear. Reaction alkahne. 



