ABSTRACTS 373 



LABORATORY TECHNIQUE 



An Eye-Shade for Use with the Microscope. E, Kellert. (Jour. 



A. M. A., 1916, 66, 1023-1024.) 



A device for attachment to the draw-tube of the microscope is de- 

 scribed. It is so designed as to prevent diffused hght from entering 

 the eye above the ocular. — G. H. S. 



An Apparatus for Filling Vaccine Ampoules. R. G. Davis, U. S. Naval 



Med. Bulletin, 1916, 10, 311-313. 



By the use of this apparatus which is briefly described and figured, 

 and which can be made in any laboratory it is claimed that ampoules 

 may be filled with vaccine without loss of time or vaccine. — E. B. V. 



The Use of the Sand Tube in Isolating the Bacillus typhosus. M. D. 



Levy. (Journ. A. M. A., 1916, 66, 1022-1023.) 



A pipette 33 cm. long and 5 to 6 mm. in diameter is bent in a U shape. 



Sand is placed in one arm to a height of 10 cm. and the other arm 

 is filled with hot bouillon. The bouillon is inoculated and the tube 

 incubated for 18 hours. 



Motile bacilli, such as Bacillus typhosus or occasionally Bacillus coli, 

 penetrate through the sand and may be isolated from the bouillon 

 above the sand. — G. H. S. 



On a Rapid Method of Cultivating the Gonococcus. Wm. B. Wherry 



and Wade W. Oliver. (Lancet Clmic, 1916, 115, 306.) 



The authors found that gonococci from the urethral pus of a boy, 



grew best on Martin's pleuritic agar, under partial oxygen tension. 



Tubes similarly inoculated and grown aerobically, yielded no growth. 



The partial oxygen tension was secured by attaching the culture tubes 



inoculated with the pus containing gonococci, to similar tubes inoculated 



with Bacillus subtilis. When isolated in this way the gonococci can 



not be subcultured aerobically, but partial tension subcultures grow 



promptly. — 0. B. 



A New Method of Separating Fungi from Protozoa and Bacteria. N. 



EIopelop'f, H. C. Lint and D. A. Coleman. (Bot. Gaz. 1916, 61, 



247-250.) 



The dilution method followed by the peculiar manner of plating, 

 makes it possible to separate fungi from bacteria and protozoa. 



As the result of this separation it has been possible to eliminate 

 fungi from experiments involving the effect of protozoa on bacterial 

 activity, by making a sub-culture from the fungi-freed solution of 

 bacteria and protozoa. 



In view of the fact that fungi are capable of producing ammonia, 

 their presence may introduce a factor not accounted for in measuring 

 the effect of soil protozoa on soil bacteria. — J. T. E. 



