420 EDWIN S. GOOD AND WALLACE V. SMITH 



shown by this sow before aborting was that she did a great 

 deal of rooting a day or two before slipping her pigs. As soon as 

 she aborted she ceased rooting. The aborted fetuses, while 

 quite well developed, were not haired over. Stained slides were 

 made from the contents of the different organs. Streak dilutions 

 were made of the heart, liver and stomach contents of each pig, as 

 well as of the afterbirth, on 2 per cent agar poured in petri dishes 

 and solidified. Some of the dishes were incubated in the air, 

 while others were cultured according to Nowak. These fetuses 

 were numbered 1 to 5. The organism with which this sow was 

 injected was obtained from the heart, liver and stomach of pig 

 no. 1; from the heart of pig no. 2; from the heart, liver and stom- 

 ach of pig no. 3; from the heart and liver of pig no. 4; and from 

 the heart and stomach of pig no. 5. Streak dilutions of these 

 organs grown in the air showed no growth at the end of twenty- 

 four hours. After forty-eight hours, however, the growth was 

 distinctly visible to the naked eye, and at the end of seventy- 

 two hours it was abundant. The streak dilutions grown under 

 diminished oxygen (Nowak method) showed no more growth 

 than that obtained in the air. Upon microscopical examination 

 of the contents of the stomachs of pigs no. 1 to 5, it was seen that 

 these organs harbored the germs in exceedingly large numbers. 

 On February 29, 1916, a pregnant sow (no. 2) was fed in ship- 

 stuff the growth of the organism, obtained from the aborting 

 sow, on two large agar slants washed off with 40 cc. of sterile 

 normal salt solution. This sow was kept in an inclosure separate 

 from sow no. 1. On March 10, 1916, she received the contents 

 of five small agar tubes in a similar feed. On March 17, the 

 attendant informed us that this sow was going to abort because 

 she was acting like the other sow, previous to aborting, in vigor- 

 ously rooting the ground floor of her pen. On March 19, nine- 

 teen days after being fed the initial dose of the organism, the 

 sow aborted. We obtained three of the pigs. She had eaten 

 the afterbirth, and in all probability had also eaten some of the 

 pigs, as she had bitten out a large piece from the side of one 

 of the pigs secured. After a long series of dilutions, we were 

 able to isolate the original organism from the stomach contents 



