422 EDWIN S. GOOD AND WALLACE V. SMITH 



causing the disease of infectious abortion in the cow, was isolated 

 from the afterbirth of an aborting sow and from the contents of 

 the umbihcus, heart, hver and stomach of two aborted fetuses. 



4. The strain of Bacillus abortus isolated from the sow responded 

 to all the biological and physiological tests of the strains isolated 

 from the uterine exudate of aborting cows by this laboratory, 

 with the exception that the original culture grew in the air 

 after the first generation. 



5. Pregnant sow no. 1, inoculated intravenously with 2 cc. 

 of an agar slant culture of the bacillus secured from the abort- 

 ing sow, washed off with 5 cc. of normal salt solution, aborted 

 five fetuses seventeen days after the injection, and the organism 

 was isolated from the afterbirth and internal organs of the fe- 

 tuses. The bacillus in this instance grew directly from the tis- 

 sues under strictly aerobic conditions. Pregnant sow no. 2, on 

 being fed the organism derived from the aborting sow, aborted 

 nineteen days afterwards. The sow ate the afterbirth and pre- 

 sumably some of the pigs. The organism was secured from the 

 stomach contents of one of the fetuses obtained. 



6. The blood serum of each of these sows, after aborting, com- 

 pletely agglutinated a strain of Bacillus abortus (Bang) derived 

 from an aborting cow, in a dilution of 1 : 100. The complement 

 was fixed in each case with 0.02 cc. of the serum. The serum 

 of a normal hog did not agglutinate in any dilution, nor did it 

 fix the complement. 



