510 C. A. LAUBACH, J. L. RICE AND W. W. FORD 



mealy consistency. The same pigment appears in the medimn. 



Glucose litmus agar slant. The growth is fairly profuse and 

 similar to that on agar. The reaction is first acid with a return 

 to alkalinity after a considerable period. 



Glucose litmus agar colonies. The colonies are similar to those 

 on agar but more profuse. Reaction of medium acid. 



Gelatin stab. Very rapid liquefaction along the line of inocu- 

 lation. The medium is entirely liquefied within 48 hours and 

 acquires an intense yellow-green color. A scum of the same color 

 is formed. 



Gelatin colonies. The colonies rest in cup-like excavations 

 caused by liquefaction. They have a definite greenish center 

 surrounded by a net-work of fine interlacing, clear, thread-like 

 filaments. The medium assumes the characteristic fluorescence. 



Broth. Profuse, flocculent growth with the production of a 

 slight green-yellow coloration. 



Peptone. Growth more pronounced than in broth and the 

 coloration more intense. A scum is also formed. 



Potato. Profuse, moist, mealy, yellow-green growth with a 

 coloration of the medium. 



Litmus milk. Slight reduction of the litmus with a suggestion 

 of peptonization within 24 hours. A precipitation of the pro- 

 teins occurs within 48 hours and peptonization progresses rap- 

 idly. A scum is formed and the fluid remaining is deeply col- 

 ored with the characteristic pigment. 



Blood serum. Moist, profuse, dull and slightly greenish-yel- 

 low growth. No liquefaction. 



Fermentation tubes. Glucose: profuse flocculent growth with 

 the formation of yellow-green scum in the bowl and a turbid 

 growth in the closed arm. The usual fluorescence is present. A 

 definite acidity is produced. 



Saccharose: the growth is identical with that in glucose but 

 no acidity is produced. 



Lactose: the growth is the same. Reaction unchanged. 



Thermal death point. The spores survive 12 pounds pressure 

 in the autoclave but are destroyed by 15 pounds. They are 

 also destroyed by one hour's exposure to steam in the Arnold 

 sterilizer. 



