522 C. A. LAUBACH, J. L, RICE AND W. W. FORD 



Gelatin colonies. Small fine colonies visible only after 48 hours. 

 Under low power they are round, regular and pale yellow with en- 

 tire margins. As they grow older they become denser and more 

 opaque and under low power show central nuclei with pale mar- 

 gins. Colonies two weeks old are thick brownish non-spreading. 

 No liquefaction. 



Broth. Turbidity and rather faint sediment. No scum. 



Peptone. Turbidity with shght sediment. No scum. 



Potato. Rather scanty yellowish moist growth becoming brown 

 in old cultures. 



Litmus milk. No change in appearance of the milk in the first 

 few days then a gradual reduction of the Htmus, completed in 15 

 to 18 days. No peptonization even in milk cultures kept under 

 observation four weeks. No coagulation. 



Blood serum. Dry thin yellowish growth becoming pale yellow- 

 brown in old cultures. No Uquefaction. 



Fermentation tubes. Glucose: turbidity in bowl, no scum, arm 

 clear. Reaction alkaUne. 



Saccharose: reaction the same. 



Lactose: reaction the same. 



Thermal death point. The spores resist steaming in the Arnold 

 sterihzer 15 minutes but are destroyed in 30 minutes. They resist 

 a pressure of 14 pounds in the autoclave but are destroyed by 16 

 pounds. / 



Bacillus brevis Migula 1900 



This organism was first described by Fliigge (1894) as Bacillus 

 No. I. According to Neide (1904) who has given an accm^ate ac- 

 count of its morphology and cultural reactions under the name 

 Bacillus lactis Fliigge, it is probably identical with Bacillus 

 cylindrosporus Burchard (1898). The correct name is that of 

 Migula. Three different strains have been isolated from milk, 

 from soil, and from dust. They agree in their cultural reactions 

 with the description given by one of us (W. W. F.) of a culture iso- 

 lated in Montreal. 



Morphology. Bacilh with pointed ends and sUghtly granular pro- 

 toplasm, sometimes fusiform in shape . In 24 hour cultures on plain 



