PREPARATION OF CULTURE MEDIA FROM WHOLE BLOOD 617 



B. typhi, B. bipolaris-septicus, B. diphtheriae, B. anthracis, B. 

 tuberculosis (isolated from a bear), B. abortus, B. pyocyaneus, 

 B. mallei, fungi, saccharomyces, etc. In all instances where 

 there was a noticeable difference in growth on the two types of 

 media, it was in favor of the blood preparation, such organisms 

 as streptococci and B. diphtheriae growing with special luxuriance. 



A test conducted on a lot of the medium prepared without 

 the addition of peptone demonstrated the fact that it is possible 

 to grow organisms such as streptococci, etc., on the peptone free 

 medium but not so luxuriantly as when peptone is added. 



In most instances the addition of glycerin is unnecessary; 

 organisms which are usually grown on glycerin-agar, will, as a 

 rule, grow very satisfactorily on plain agar prepared from the 

 blood bouillon. 



This medium, as can be seen from the method outlined above, 

 is very easy to prepare, in fact more simple of preparation than 

 the ordinary beef infusion, as the time required for trimming 

 away fat, fascia, etc., is eliminated. This fact, together with 

 the small cost and the adaptability of the medium for organisms 

 which do not thrive well on the ordinary culture media should 

 recommend it as a valuable product in the laboratory. It can 

 practically take the place of serum-agar which is somewhat 

 more difficult to prepare. 



An effort was made to produce a concentrated extract from 

 the blood, similar to beef extract. By considerably reducing 

 the amount of distilled water added to the cut up blood clot 

 and serum, a much more concentrated extract is obtained and 

 this can be reduced to the desired consistency through evapora- 

 tion over a water bath. Media prepared from this concentrated 

 extract proved satisfactory in every way. 



A concentrated extract from blood can also no doubt be pre- 

 pared by the method used in packing houses for the production 

 of meat extracts, i.e., through the use of heat and vacuum. 



REFERENCES 



LiCHTENSTEiN, S. 1916 Centralbl. f. Bakt. Abt. I. Orig. 77, 362-363. 

 ScHMiTZ, K. E. F. 1916 Centralbl. f. Bakt. Abt. I. Orig. 76, 306-320. 



SzASZ, A. 1915 a Centralbl. f. Bakt. Abt. I. Orig. 75, 489-495. 



SzAsz, A. 1915 b Centralbl. f. Bakt. Abt. I. Orig. 77, 111-12. 



