ABSTRACTS 



687 



Hepatic Baderiolysins. W. H. Manwaring and Harry C. Coe. 



(Preliminary Report). Proc. Soc. Exp. Biol, and Med., 1916, 13, 



177. 



If pneumococci are deposited by perfusion methods in the liver of a 

 normal rabbit in the presence of normal rabbit blood and the liver then 

 incubated at 37°C., the bacteria multiply and overgrow the liver after 

 six hours. If the analogous experiment is tried with liver and blood 

 of an immune rabbit a gradual decrease in the deposited pneimiococci 

 is observed. The bacteria in the larger vessels, not in contact witli 

 parenchyma, are not destroyed. There is apparently an hepatic 

 mechanism in the immune animal for the destruction of microoi-gan- 

 isms.— W. J. M. 



Protein Absorption by Blood Corpuscles. W. H. Manwaring and 

 YosHio KusAMA. Proc. Soc. Exp. Biol, and Med., 1916, 13, 173. 

 Freshly drawn defibrinated rabbit's blood added to a 1 per cent 

 solution of goat's serum and incubated for one hour shows only 25 

 per cent of the goat protein remaining in the fluid portion. If the 

 serum and corpuscles are now separated and allowed to undergo inde- 

 pendent autolysis (10 hours at 37°C.) a distinct restoration of the 

 goat protein occurs in both, but is more pronounced in the corpuscle 

 fraction. Analogous results may be obtained in vivo, indicating that 

 parenterally introduced proteins are absorbed in a large measure by 

 the circulating blood corpuscles. — W. J. M. 



Analysis of the Anaphylactic and Immune Reactions by Means of the 

 Isolated Mammalian Heart. W. H. Manwaring, Arthur R. Mein- 

 HARD AND Helen L. Denhart. Proc. Soc. Exp. Biol, and Med., 



1916, 13, 175. . . ^ u f ■ 



The heart of a rabbit sensitized to goat serum, tested by perfusion 

 with 7 to 10 per cent goat serum is more resistant than a normal heart. 

 Hearts of rabbits sensitized or immunized by repeated injections are 

 still more resistant. Normal rabbit serum, corpuscles or defibrinated 

 blood, added to the perfusion fluid decreases its toxicity. Anaphy- 

 lactic' rabbit serum similarly added increases the toxicity. The ac- 

 tive principle responsible for this effect is destroyed by heating to 

 60°C for 30 minutes and is not restored by the addition of fresh nor- 

 mal serum, and is therefore not complement and probably not precipi- 

 tin Such inactivated anaphylactic sera are strongly antitoxic. 



— W. J. M. 



A Method of Producing Antigen for Complement-Fixation in Tubercu- 

 losis. H. R. Miller and Hans Zinsser. Proc. N. Y. Pathol. 



Soc," 1916, 16, 28. , • , -^u u 



A weighed amount of bacillary substance is ground up with salt 



and subsequently suspended in distilled water sufficient to give isoto- 



nicity. Such antigen is not anticomplementary in quantities of 1.0 



