ABSTRACTS 693 



cilli were more resistant to infection than those in which salt solution 

 or normal serum was substituted for the tubercular serum. 



The results obtained after infecting pigs which had been actively 

 immunized with von Ruck's vaccine were not entu-ely satisfactory. 

 On the whole, however, it appeared that animals possessing an active 

 immunity showed a greater resistance to infection than control ani- 

 mals.— M. W. C. 



LABORATORY TECHNIQUE 



Culture Media for Paramecia and Euglena. R. M. Strong. Science 



1916, 44, 238. 



The author describes a simple and convenient medium for the abun- 

 dant production of Paramecia and Euglena for class work. — C. M. H. 



Another Use of the Double Plate Method. W. D. Frost and Freda M: 



Bachmann. Science, 1916, 44, 433. 



The senior author justly insists upon his priority in the use of the 

 double plate method, introduced by him for the study of antagonism 

 towards the Bacillus typhi in 1904. In the present work on anti- 

 septic values of certain spices and condiments, he describes a modi- 

 fication of his method, made by substituting semi-discs of muslin for 

 glass rods in the petri dishes. When the plain agar has solidified in 

 one-half of the plate, the cloth is removed and the agar containing the 

 condiment is poured into the other half. — C. M. H. 



Studies on Laboratory Media. Jane L. Berry. Collected Studiesi, 



Bureau of Laboratories, New York City, 1914-1915, 8, 288. 



A medium containing the usual amount of agar and one-twelfth 

 the amount of meat-extract, peptone and salt gave good results in 

 milk plates, with much reduced cost. Still better results for milk 

 work were given by agar made with a one to fifteen dilution of Hot- 

 tinger's stock broth. This is also good for carrying stock cultures. 



Agar which has been used once for cultivation of various organisms 

 can be melted, poured together, titrated, sterilized, and used over 

 again, especially where large numbers of bacteria are desired as in 

 vaccine work. 



Tests made on several American-made peptones compared favor- 

 ably with Witte's peptone. — T. G. H. 



A New Culture Medium for the Isolation of Bacillus Typhosus from 

 Stools. J. E. Holt-Harris and Oscar Teague. Jour. Infect. 

 Diseases, 1916, 18, 596-600. 



The authors point out that the chief difficulty in the use of the 

 Endo plate is that colonies of B. coli may redden the entire plate and 

 thus obscure typhoid colonies. They recommend a medium com- 

 posed of nutrient agar containing peptone, sodium chloride, Liebig's 

 meat extract, sucrose, lactose, eosin and methylene blue. On such 



