44 R. E. BUCHANAN 



Obligate parasites. Cells usually elongate and fusiform. Gram- 

 negative? Anaerobic. Non-motile. No spores. In some respects 

 approaching the spirochetes in morphology. 



The type species is Fusiformis termitidis Hoelling (possibly 

 F. dentium). 



Vuillemin (1913, p. 521) has proposed that the name Man- 

 tegazzaea Trevisan be retained as one of the ''formogenera con- 

 servanda," with the fusiform bacillus of the medical writers as 

 the type species. The name as used by Trevisan was used 

 principally for certain of the sulphur bacteria, and not for forms 

 related to this type. 



Subtribe II. Hemophilinae Subtrib. nov. 



Strict parasites, requiring hemoglobin or at least serum for their 

 growth in media. Gram-negative. N on-motile. Cells may be pleo- 

 morphic. Usually very small. No spores. 



The two genera may be differentiated by the following key: 



Key to the genera of Hemophilinae 



A. Requiring serum for growth. Cells almost ultra-microscopic. Stain best 



by Giemsa. Involution forms characteristic Genus 1. Aslerococcus 



B. Requiring hemoglobin for growth. Stain readily with ordinary aniline 



dyes. Involution forms infrequent Genus 2. Hemophilus 



Genus I. Asterococcus Borrel, et al., 1910, p. 179 



Pleomorphic cells, appearing at different stages of development 

 as isolated cocci or as chains of cocci, as rods and as filaments 

 variously branched and swollen. Very minute, almost ultra- 

 microscopic. Non-motile. No spores. Stains with difficulty, best 

 with GKemsa stain. Growth in cultures only in presence of serum 

 or oj hemoglobin. 



The type species is Asterococcus mycoides Borrel, et al., of bovine 

 pleuropneumonia. 



Genus 2. Hemophilus. Committee 



Rod-shaped cells, minute, non-motile, without spores, strict 

 parasites, growing best or only in presence of hemoglobin. Gram- 

 negative, stain readily with aniline dyes. 



