74 FRED W. TANNER 



Capsules were demonstrated on the following cultures by 

 means of Welch's method as described by Muir and Richie 

 (1913). 



2, 3 Ps •• 221.2223133 



6 Ps 221 . 2232133 



14 Ps 221 . 2332133 



22 Ps 221 . 2222132 



26 Ps 221 . 2233133 



37, 75 Ps 221 . 2333133 



46 Ps . 221 .2332133 



50 Ps 221 . 2223133 



60 Ps 222.2223132 



6S Ps 222.2233133 



79 Ps .' 222.2223133 



The broth cultures of these strains were invariably viscid. 

 The group numbers are quite similar, but differ slightly in a 

 few characteristics. All but three strains liquefied gelatin. 

 These strains gave slimy growth in most media. 



Growth on agar stroke 



All strains were grown on this medium and produced a dis- 

 tinct fluorescence. Abundant growth was secured at 37 °C. 

 One strain secured from another laboratory refused to grow at 

 37°, but grew well at 20°. This strain, however, was not in- 

 cluded. Strain 100 was peculiar in that the fluorescent color was 

 followed by a permanent red. This may have been due to an 

 acid reaction of the medium. 



Growth of potato slants 



The potato slants were made in the usual way. In almost 

 every case the growth was spreading and was either moderate 

 or abundant. At the end of about two days incubation it was 

 limited to the hne of inoculation, but spread rapidly after longer 

 incubation. The medium was turned to a dark brown or green. 

 Observations were made at the end of ten days, when the potato 

 and the water in the bottom of the tube were poured into a 

 mortar and tested for starch destruction. 



