82 FRED W. TANNER 



mode is also secured in the distribution curve for lactose. Here, 

 again, a single type of bacteria is indicated. With regard to the 

 action on glycerol, however, two modes are evident which may 

 indicate two types of bacteria. In general, these 100 strains of 

 fluorescent bacteria are alike with regard to fermentation of 

 glucose and non-fermentation of sucrose, lactose and glycerol. 

 Edson and Carpenter (1912) state that with the fluorescent 

 bacteria from maple sap no sharp line of differentiation exists 

 between acid production and no acid production. The distribu- 

 tion curve for the fluorescent bacteria from water indicate the 

 same thing with regard to sucrose, lactose and possibly glycerol. 



Growth in Uschinsky^s inediuin 



This medium was made up after the following formula and 

 sterilized in the Arnold on three successive days: Distilled water, 

 1000 cc; glycerol, 35 grams; sodium chloride, 6 grams; calcium 

 chloride, 0.1 gram; magnesium sulfate, 0.35 gram; dipotassium 

 phosphate, 2 grams. 



Vigorous growth was secured in this medium with all strains 

 except no. 10. In many cases the medium was strongly fluo- 

 rescent. A pellicle was formed by many strains and many of 

 them exhibited a very viscid growth. 



Growth in FrankeVs medium 



This medium was prepared after the following formula: so- 

 dium chloride, 5 grams; monocalcium phosphate, 2 grams; am- 

 monium lactate, G grams; asparagin, 4 grams; distilled water, 

 1000 cc; N NaOH, 20 cc. 



The medium was filtered and sterilized in the Arnold steril- 

 izer on three successive days All strains grew abundantly and 

 produced much pigment. 



Growth in Sullivan's medium 



This medium (SuUivan (1905) ) which was found to support 

 good pigment formation was made up as follows: distilled water, 



