146 



H. J. COKPER AND H. C. SWEANY 



1 . HCl to 0.1 per cent 



2. HCl to 0.1 per cent and 0.1 per cent 



acid caseinate 



3 . Neutral control 



4. 3 cc. 0.1 per cent nucleic acid 



INTERVALS 



mgm 

 0.020 



0.022 

 0.018 

 0.024 



36 



HOURS 



mgm. 



0.026 



0.039 

 0.027 

 0.025 



4 



DAVS 



0.027 



0.043 

 0.036 

 0.028 



7ngm. 



0.031 



0.048 

 0.046 

 0.034 



mgm.. 



0.039 



0.051 

 0.053 

 0.041 



II 



DATS 



mgm. 



0.042 



0.058 

 0.065 

 0.043 



Series V. Urease 



The presence of urease in tubercle bacilli was determined by 

 making an emulsion of human bacilli in physiological salt solu- 

 tion, 3 cc. bacillary residue made up to 32 cc. in each of two 

 tubes, and adding 50 mgm. of urea and 5 cc. toluene to a heated 

 control and a test. After withdrawal of a 3 cc. control (filtering 

 tlirougn%ard filter paper and using 2 cc. for analysis) the tubes 

 were incubated at 37°C., and samples taken out for analyses at 

 various intervals. The amount of urea decomposed was deter- 

 mined by the Folin aeration method. The enzyme action was 

 stopped by the addition of 1 cc. saturated sodium carbonate to 

 2 cc. of the filtrate. The results obtained are expressed as milli- 

 grams of nitrogen in 2 cc. of filtrate. 



Summary. Tubercle bacilli possess an enzyme capable of 

 decomposing urea. 



Series VI. Diastase and Invertase 



The presence of diastase and invertase in the tubercle bacillus 

 was tested by determining the amount of reducing sugar formed 

 from starch and sucrose as performed by Meyers and Rose 



