BACT. PERTUSSIS AND B. BRONCHISEPTICUS 201 



than 1-20, it also agglutinated Bad. pertussis and generally in 

 higher dilutions. In one instance when there was no agglutina- 

 tion against B. bronchisepticus, the serum agglutinated Bact. 

 pertussis in a dilution of 1-400 (rabbit E) (see table 4). 



A few of these rabbits subsequently developed symptoms of 

 distemper; the others may have recovered from an attack. 



Sera from only two distemper dogs have been tested, but, 

 with these, similar results were obtained. As in rabbits, agglu- 

 tinins for Bact. pertussis were manifest in higher dilutions than for 

 B. bronchisepticus. These dogs exhibited typical symptoms of 

 distemper and were in the later stages of the disease. 



Dog 1 agglutinated B. bronchisepticus no. 36 (dog) at 1-20; 

 and Bact. pertussis no. 0363 (Bordet), 1^00 and no. 93, 1-1000. 

 Dog 2 agglutinated B. bronchisepticus no. 36 (dog) at 1-80; 

 and Bact. pertussis no. 0363, 1-1000 and no. 93, 1-1000. 



On absorption with B. bronchisepticus the agglutinins for that 

 oj"ganism are removed, while the agglutinins for Bact. pertussis 

 are affected little or not at all. On absorption with Bact. 

 pertussis, the pertussis agglutinins are removed, while those for 

 B. bronchisepticus are unaffected. 



ABSORPTION REACTIONS 



Upon submitting to absorption tests those antiserums which 

 were produced by the injection of B. bronchisepticus antigen into 

 rabbits, and which were found to agglutinate both the B. bronchi- 

 septicus and Bact. pertussis suspensions, the following results 

 were obtained. 



Upon absorbing with B. bronchisepticus suspension, the agglu- 

 tinins for 5. bronchisepticus (the major agglutinins) were absorbed, 

 but the Bact. pertussis agglutinins (the minor agglutinins) were 

 still intact (table 5), and an absorption with Bact. pertussis was 

 necessary before they were neutralized. In other words, the 

 B. bronchisepticus antigen stimulated the formation of both B. 

 bronchisepticus and Bact. pertussis agglutinins, but contrary to 

 what one would expect, was not able to absorb the Bact. pertussis 

 agglutinins, (the minor agglutinins). It was also found, in the 



