BACT. PERTUSSIS AND B. BRONCHISEPTICUS 



203 



case of dogs suffering with distemper, that the serum agglutinated 

 Bad. pertussis antigen in higher dilutions than the B. bronchi- 

 septicus antigen. Absorption with B. bronchisepticus antigen 

 took out only the B. bronchisepticus agglutinin, while it was 

 necessary to absorb with Bact. pertussis antigen before the Bact. 

 pertussis agglutinin was neutralized (see table 7). The Bact. 

 pertussis agglutinins, therefore, were fixed or stable, as far as the 

 B. bronchisepticus was concerned, but absorbable for the Bact. 

 pertussis, and this type of an agglutinin, which can be produced 



TABLE 5 



by one antigen to be taken up or absorbed more readily by 

 another, has been called by the writers, for the lack of a better 

 term, a transitive agglutinin. 



The antisera were, identical to those used for the agglutina- 

 tion tests. 



The heavy suspensions used for absorption were made from twenty 

 hour growths on plain agar in whiskey flasks, suspended in .85 per cent 

 salt solution to which had been added 0.5 per cent formalin. About 

 10 cc. salt solution was used to a flask for B. bronchisepticus and 4 cc. 

 for Bact. pertussis. The suspensions were shaken over night in a 

 mechanical shaker and then strained through mull. Each strain had 



