284 



PHILIP HADLEY 



ferred to tubes of chicken broth and incubated at 37°C. for 

 ninety-six hours. No growth appeared at the end of that time. 

 In the meantime the remaining filtrate was divided into three 

 portions: portion a was placed in the ice-box; portion b was 

 incubated for twenty-four hours at 37°C., and portion c was 

 heated at 62°C. for twenty minutes. The incubated filtrate 

 showed no growth. It was intended to inject all three portions 

 into rabbits, but, owing to an accident, only the ice-box filtrate 

 and the heated filtrate were employed for rabbits. These were 

 injected intravenously in amounts of 1 cc. as shown in the 

 accompanying table. Of the incubator filtrate 1 cc. was injected 

 intravenously into an adult hen: 



From these results there is no evidence of a toxic filtrate from 

 the growth of B. avisepticus. 



The tests described above were made with filtrate from a forty- 

 eight-hour broth culture. Assuming that sufficient time might 

 not have been given -for toxin-formation, the second flask was 

 incubated for four days at 37°C., and the culture passed through 

 a Berkefeld N-candle under suction. The fifteen-minute filtrate 

 was tested for sterility by the inoculation of five tubes of chicken 

 broth with 0.1 cc. samples. No growth resulted after forty-eight 

 hours' incubation. The remaining filtrate was divided into two 

 lots. One was placed in the incubator and the other in the ice- 

 box for about twenty-four hours, until used. Intravenous inocu- 

 lations were made as shown in the following tabulation: 



