288 



PHILIP HADLEY 



The culture grew well on chicken agar ; resembled in all respects 

 growth of Bad. pullorum or B. typhosus. In broth there was a 

 fair clouding at the end of twenty-four hours. 



No gas was formed in glucose, sucrose or lactose (Smith tubes). 

 Titration of culture from the open arm of these cultures, and of 

 cultures in other sugar broths (in plain tubes) at the end of 

 five days gave the following results: 



Litmus milk inoculated with one loopful of a twenty-four-hour 

 broth culture showed a slight acidity in twelve hours at 37°C. 

 This increased slightly for the next twelve to twenty-four hours 

 but after thirty-six to forty-eight hours there was a tendency to 

 return to neutral. The cream ring, however, began to turn blue* 

 about twenty-four hours after the inoculation of the tubes and 

 the color steadily increased so to as give a blueness of grade 2^ 

 before the body of the milk had returned to neutral. Neutrali- 

 zation occurred on the sixth day after inoculation. On the sev- 

 enth the body of the milk as well as the cream ring was slightly 

 alkaline and on the eighth distinctly alkaline (grade 1). Grade 

 3 alkaline was attained on the thirtieth day. 



Without entering into further details regarding the biochemical 

 reactions, a sufficient number of data have been presented to 

 indicate clearly that the culture with which Dr. Bull worked was 

 not B. avisepticus in the sense that this is the causative agent in 



* It is interesting to note that in alkalining cultures such as B. pullorum, B. 

 gaUinarum and the paratyphoids A and B, the alkalinity may be shown in the 

 cream ring for some days before the color of the body of the milk has even returned 

 to neutral, and many daj's before the body of the milk becomes alkaline. 



'SeeHadley (1917). 



