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O. W. HUNTER 



inoculating the tubes of disinfectants and for transferring the 

 organisnls from the disinfectants to the culture media. Sterile 

 litmus milk was used as the culture medium. 



The disinfectants employed were: washing soda, calcium 

 hypochlorite, lime, boric acid, cresol and carbolic acid. The 

 vitality of the exposed cultures was determined by incubating 



TABLE 2 

 Resistance towards disinfectants 



Growth +; no growth — . 



the litmus milk tubes for four days at 37° and noting the presence 

 or absence of growth. 



The results are noted in table 2. 



The results in table 2 indicate that washing soda exhibits little 

 disinfectant action on the yeast. Exposure for two hours to both 

 1 and 2 per cent solutions failed to kill the organism, while a 5 

 per cent solution prevented their growth in seventy-five minutes. 



Calcium hypochlorite prevents the development of the yeast 

 in 2 and 5 per cent solutions within one minute, while a 1 per 

 cent solution acts similarly in three minutes. 



