BACT. PERTUSSIS AND B. BRONCHISEPTICUS 



311 



The results of the complement fixation tests may be seen in the 

 following table: 



B.bronchisepticus (dog) no. 

 123 



B. bronchisepticus (mon- 

 key) no. 123 



B. bronchisepticus (human) 



Bact. pertussis no. 0363 



Bact. pertussis no. 109 



Bact. pertussis no. 248 



;!. a 



+ 

 + 

 + 

 + 

 + 



+ Denotes complete inhibition of hemolysis. 



— Denotes incomplete or no inhibition of hemolysis. 



It was found in a large majority of the tests as represented in 

 the chart, which is a composite, that the B. bronchisepticus 

 immune serum bound the complement in the presence of both 

 the bronchisepticus and pertussis antigens, while the B. pertussis 

 immune serum bound the complement in the presence of the 

 homologous antigen and also the human and monkey strains of 

 B. bronchisepticus. It did not bind the complement in the pres- 

 sence of a dog strain of B. bronchisepticus. 



SUMMARY 



1. B. bronchisepticus immune serum bound the complement in 

 the presence of both B. bronchisepticus and Bact. pertussis antigen. 



2. Bact. pertussis immune serum bound the complement in 

 the presence of Bact. pertussis antigen and B. bronchisepticus 

 antigen of both human and monkey origin but not of dog origin. 



3. The complement fixation test is not a reliable method of 

 differentiating between the two organisms in question. 



4. Bacterial autolysates were found to be the most stable and 

 satisfactory antigens. 



