392 



NATHAN HERMAN AND LEO F. RETTGER 



TABLE 1 

 Showing the effect of glucose on nitrogen metabolism 



ORGANISM 



Control uninoculated. 



B. subtilis 



B. ramosus 



P. vulgaris 



P. mirabilis 



Staph, aureus 



Staph, aureus, II 



Staph, albus 



B. typhi 



B. paratyphi A 



B. coli 



B. diphtheriae 



REACTION 



4.0 

 2.0 

 6.0 

 4.0 

 2.0 

 2 

 2.0 

 4.0 

 0.0 

 2 

 2.0 

 4.0 



D 



4.0 



6.0 



4.0 



12.0 



14.0 



10.0 



10.0 



10.0 



14.0 



14.0 



20.0 



6.0 



SORENSEN 



4.0 



22.0 



14.0 



8.0 



8.0 



4.0 



8.0 



16.0 



6.0 



6.0 



4.0 



6.0 



D 



4.0 

 28.0 

 8.0 

 6.0 

 6.0 

 6.0 

 6.0 

 4.0 

 4.0 

 6.0 

 4.0 

 6.0 



C 



1.0 



0.0 



0.75 



0.0 



0.0 



0.7 



0.7 



0.7 



0.8 



0.8 



0.85 



0.85 



D 



1 

 0.0 

 1.0 

 1.0 

 1.0 

 1.0 

 1.0 

 1.0 

 1.0 

 1.0 

 1.0 

 1.0 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 



The composition of Media C and D is as follows ; 



Medium C 



Medium D 



per 

 cent 



Peptone 0.5 



NaCl 0.25 



Peptone. 

 NaCl... 

 Glucose. 



per 

 cent 



.0.5 



.0.25 



.1.0 



A more extensive study of the influence of carbohydrates on 

 the protein metaboUsm of Bacillus subtilis was then conducted. 

 Five different media were prepared as follows : 



Medium 1 was used as control. The five solutions were trans- 

 ferred to test tubes (10 cc), each tube but the control being 

 inoculated with a pure strain of Bacillus subtilis. A series of 

 fifteen tubes was used for each medium. Tubes from each se- 

 ries were taken from the incubator on alternate days, and tested 



