NITROGEN METABOLISM OF BACTERIA 401 



CONCLUSIONS 



1 The presence of a carbohydrate in a culture medium may 

 inhibit protein metabohsm, depending on the nature of the 

 medium and on the type of the organism. 



2. The presence of sufficient buffer in a medium encourages 

 continued normal nitrogen metabolism. 



3 A utilizable carbohydrate in a medium has a true ^protein 

 sparing' effect providing the hydrogen ion concentration is 

 maintained within suitable limits. 



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