444 I. J. KLIGLER 



of a slant of a killed culture and ending with 1/lOth of the living 

 culture. 



The results with the sera prepared from isolated colonies of 

 each bacillus coincided with those of the original test. The fact 

 should be mentioned that the particular Shiga immune serum 

 was without agglutinating action on other Shiga cultures or 

 cultures of the Flexner dysenteric group. Table I brings out 

 in brief form the points just described. The cross-agglutinat- 

 ing colon bacillus culture is number 13 and the Shiga bacillus cul- 

 ture number 30. 



Absorption of the immune sera was carried out with the homol- 

 ogous and heterologous agglutinating cultures, after which 

 agglutination tests were made. The results are given briefly 

 in table 2. 



SUMMARY 



Two cultures are described, one a typical B. coli and the other 

 a typical B. dysenteriae, Shiga, culture, which yield immune sera 

 possessing agglutinating properties of practical equal quantity 

 for each culture. Absorption experiments made with each cul- 

 ture upon each kind of immune serum indicate that two distinct 

 agglutinins are yielded in about equal amount in the process of 

 immunization of rabbits with the respective cultures. The two 

 agglutinins are specific ones, each for its own culture, and acces- 

 sory (paragglutinin) , each for the other culture. The absorption 

 of the accessory agglutinin leaves the specific agglutinin quanti- 

 tatively unaffected. 



The significance of this reciprocal agglutinative property in 

 respect to the two bacilli described can only be surmised. The 

 obvious suggestion is a group relationship between certain 

 strains of colon and dysenteric bacilli, a subject hardly to be 

 pursued profitablv in this connection. 



REFERENCES 



Castellani, a. 1902 Ztschr. f. Hyg., 40, 1. 



Park, W. H., and Williams, W. 1910 Pathogenic Microorganisms, 166, 168. 



RoDET, A. 1897 Compt. rend, de laSoc. de Biol., 4, 874. 



Stern, R. 1898 Centralb. f. Bakt., 23, 673. 



