OZENA AND DISTEMPER 



501 



SUMMARY OP CULTURAL REACTIONS 



1. B. ozaenae may be readily differentiated from B. hronchi- 

 septicus by any one of the following reactions; by growth on 

 potato, on LoefHer's blood serum and in litmus milk, by the 

 fermentation reaction in glucose media and by the indol reac- 

 tion in Dunham's solution. 



2. Less important differentiating characteristics are found in 

 morphology, motility, colony formation and odor. 



Differentiating cultural characteristics 



Morphology 



Motility 



Colony 



Loeffler's blood serum 



Potato 



Plain broth 



Litmus milk 



Dunham's 



Fermentation 

 Glucose 



PEREZ BACILLUS 



Small coccoid bacillus, 

 no filaments 



Sluggish 



Old colony, lobate 



No proteolysis, cream to 

 greenish yellow 



Limited, faintly yellow 



Uniform turbidity, char- 

 acteristic nauseating 

 odor 



Small amount of acid 



Indol positive 



Acid — gas 



B. BKONCHISBPTICrra 



Small narrow bipolar ba- 

 cillus . Filaments in liq- 

 uid media 



Active 



Round, entire 



No proteolysis, old cul- 

 tures tan color 



Spreading, tan 



Uniform turbidity, odor 

 of stale bread 



Decided alkalinity 

 Indol negative 



Alkaline — no gas 



AGGLUTINATION REACTIONS WITH B. BRONCHISEPTICUS AND PEREZ' 



BACILLUS 



Rabbits have been treated with vaccines of the following 

 cultures of B. bronchisepiicus and Perez' Bacillus and the sera 

 obtained for the purpose of making cross agglutination tests 

 between these two organisms. 



B. bronchisepiicus no. 36, from dog 1 



B. bronchisepiicus no. 123, from monkey > Isolated by N. S. F. 



B. bronchisepiicus from human J 



Perez' Bacillus no. 1 Isolated by Ward 



Perez' Bacillus no. 2, "Hofer" strain | European strains 



Perez Bacillus no. 3, "Vienna strainj 



Perez' Bacillus no. 4 Isolated by Ward 



