502 



N. S. FERRY AND ARLYLE NOBLE 



Vacciyws. Each organism was transplanted daily for several 

 days on plain agar; then twenty-four hour growths were washed 

 off in 0.85 per cent salt solution plus 0.2 per cent trikresol (5 

 cc. per culture). Each vaccine was thoroughly shaken in a 

 mechanical shaker and two days later tested for sterility. 



Production of antisera. Before the rabbits were injected each 

 was bled from an artery in the ear and the serum tested for ag- 



TABLE 1 



+ -|--|- = Complete agglutination with all organisms clumped and fluid clear. 

 -{--\- = Partial agglutination, with marked clumping but fluid not cleared up. 

 + = Slight agglutination but still with positive clumping. 

 — = No clumping and no clearing. 



glutinins against both B. bronchisepticus and Perez' Bacillus. 

 No rabbit showed an agglutination titre of above 1 in 20 against 

 either organism. 



Each rabbit received three intravenous injections of 0.5, 1.0 

 and 2.0 cc. of killed vaccine three days apart, and was bled to 

 death on the fourth day after the last dose. To the sera was 

 added 0.2 per cent trikresol. 



