PROTEOLYTIC ENZYMES OF SOIL FUNGI 



521 



of the organism, but their activity will increase with the age of the 

 organism, since no acids or other harmful agents are produced, 

 till the organism begins to autolyze, and then the further pro- 

 duction of the enzymes may be affected by the products of 



TABLE 7 

 Influence of incubation of enzymes upon the amino nitrogen produced 



ORGANISMS USED 



Control .... 



A . niger 



A. niger 



A. niger 



A. niger 



A. niger 



A. niger 



A. niger 



A. niger 



A. niger 



A. niger 



A. niger 



A. niger 



A. niger 



A. niger 



A. niger 



A. ochraceus 

 A. ochraceus 

 A. ochraceus 

 A. ochraceus 

 A. ochraceus 

 A. ochraceus 

 A. ochraceus 

 A. ochraceus 



CULTURE MEDIUM 



Peptone 

 Peptone 

 Peptone 

 Peptone 

 Peptone 

 Peptone 

 Peptone 

 Peptone- 

 Peptone' 

 Peptone- 

 Peptone- 

 Peptone- 

 Peptone- 

 Peptone- 

 Peptone- 

 Peptone- 

 Peptone- 

 Peptone- 

 Peptone- 

 Peptone- 

 Peptone- 

 Peptone- 

 Peptone- 



-Czapek 

 -Czapek 

 -Czapek 

 •Czapek 

 -Czapek 

 •Czapek 

 •Czapek 

 ■Czapek 

 -Czapek 

 -Czapek 

 -Czapek 

 -Czapek 

 -Czapek 

 -Czapek 

 •Czapek 

 -Czapek 

 -Czapek 

 ■Czapek 

 Czapek 

 Czapek 

 Czapek 

 Czapek 

 Czapek 



AGE 



OF CUL- 

 TURE 



days 



INCU- 

 BATION 



hours 



MILLIGRAMS OP NHj=N PER 

 100 CC. OF SOLUTION 



1 per cent 

 peptone 



Exoen- 

 zyme 



21.46 



31.13 

 33.96 

 41.14 

 43.51 

 47.64 



Endo 

 enzyme 



20.30 

 24.34 

 32.26 

 42.81 

 46.90 

 52.63 

 54.90 

 61.94 

 63.60 

 28.30 

 29.43 

 40.03 

 42.38 

 52.08 

 59.72 

 61.94 

 30.00 

 33.39 

 41.14 

 45.20 

 51.52 

 53.80 

 56.96 

 67.56 



1 per cent 

 casein 



Exoen- 

 zyme 



7.30 



32.26 

 33.96 

 46.15 

 51.98 

 60.94 

 62.04 

 63.60 

 70.38 



Endo- 

 enzyme 



6.28 

 15.85 

 21.51 

 34.47 

 43.51 

 47.09 

 50.51 

 58.07 



17.55 

 22.64 

 44.48 

 50.98 

 59.28 

 74.10 

 76.31 

 30.55 

 35.66 

 57.88 

 74.02 

 84.76 



autolysis. The difference in behavior of the enzymes of A. 

 niger grown on the peptone medium may be explained by the 

 fact that the peptone and its decomposition products present in 

 the medium may exert a buffer action upon the acids of the 

 medium thus decreasing their injurious effect. 



