98 



J. T. CONNELL AND L. E. HOLLY 



TABLE 5 



K salts antigen per liter 

 K salts antigen per liter 

 K salts antigen per liter 

 acids antigen per liter 

 acids antigen per liter 

 acids antigen per liter 



1 cc. of A plus 1 cc. cell suspension 



1 cc. of B plus 1 cc. cell suspension 



1 cc. of C plus 1 cc. cell suspension 



1 cc. of D plus 1 cc. cell suspension 



1 cc. of E plus 1 cc. cell suspension 



1 cc. of F plus 1 cc. cell suspension 



These emulsions remained perfectly clear 



INACTI- 

 VATION 

 AFTER 

 ONEHOUR 

 AT 65°C 



+ +501 

 -1 hr. 

 -1 hr. 

 + +38' 

 -1 hr. 

 -1 hr. 



The question of partial digestion of the broth on the part of 

 the microorganisms during growth led us to attempt a similar 

 procedure in the effort to copy as closely as might be the germ 

 action in the production of our artificial lysin. We added a 

 small quantity of pancreatin powder to the broth containing the 

 antigen and digested the mixtures in the water bath at 45°C. 

 for one hour. The result of this experiment was complete 

 inactivation of the lysin, rather than one favorable to lysin 

 production. The question then arose as to whether the loss of 

 hemolytic power was not due to simple adsorption of the antigen 

 rather than to digestion, and the following experiment showed 

 such to be the case. 



One cubic centimeter of artificial B. megatherium hemolysin 

 containing 100 mgm. of the K salts per liter hemolyzed 1 cc. of 

 cell suspension in ten minutes. Five cubic centimeter quanti- 

 ties of this hemolysin were treated with a definite amount of 

 starch, and the same quantities with defatted colon bodies. 

 These mixtures, together with 5 cc. controls of untreated hemo- 

 lysin were placed in the water bath at 45°C. for one hour, and 

 identical specimens were placed in the ice box over night. After 



