102 J. T. CONNELL AND L. E. HOLLY 



at which inactivation occurs, (c) The natural and artificial 

 lysin of the B. megatherium is inactivated by adsorbents under 

 the same conditions. The natural streptolysin is not inactivated 

 by these agents, nor is the artificial lysin in the presence of 

 serum, (d) Analysis of the broth in which B. megatherium, had 

 been grown for twenty-four hours showed the presence of approxi- 

 mately 60 mgm. per liter of the fat complex of the organism. 

 No analysis of the Streptococcus broth was made because of the 

 serum present, (e) Neither natural or artificial streptolysin 

 has thus far yielded satisfactory antilysin. Both natural and 

 artificial lysins of the B. megatherium gave rise to strong specific 

 antilysins. 



We have shown that certain artificial specific fat complexes 

 exhibit all the reactions characteristic of the bacteria examined. 



We believe that the hemolysins of the organisms studied con- 

 sist of the respective fat antigens of the bacteria existing in 

 definite colloid states. 



REFERENCES 



Besredka 1901 Ann. de Tins. Past., p. 880. 



Bulloch and Huntek 1900 Centbl. f. Bakt., Bd. 28, p. 866. 



Ehrlich 1898 Berl. klin. Woch., p. 273. 



Levy, E., and P. 1901 Centlbl. f. Bakt., Bd. 30, p. 405. 



LuBENAU 1901 Centbl. f. Bakt., Bd. 30, p. 402. 



Nbisser and Wechsburg 1901 Zeitsch. f. Hyg., Bd. 36. 



Rtjediger 1903 J. A. M. A., 41, 962. 



Todd 1901 Lancet, 2, 1663. 



Weingeroff 1901 Centbl. f. Bakt., Bd. 29. 



