106 



C. C. WARDEN, J. T. CONNELL AND L. E, HOLLY 



culture. The antigenic titer of the control antigen was 0.04 cc. 

 and that of the artificial antigens 0.1 cc. of emulsions made by 

 mixing 1 cc. of the alcoholic antigen solutions with 16.5 cc. of 

 salt solution. Two units of amboceptor and 2 units of comple- 

 ment were used, and 0.5 cc. of a 2 per cent washed sheep cell 

 suspension, all tubes being brought to a volmne of 1 cc. with 

 salt solution. The first incubation was for thirty minutes at 

 37°C., the second for one hour followed by standing at 20°C. 

 for several hours. The serums of control and immunized rab- 

 bits were inactivated at 56°C. for thirty minutes. The results 

 of the tests are shown in tables 1, 2 and 3. 



TABLE 1 

 First group of rabbits 



1. Rabbit injected organisms 



2. Rabbit injected organisms 



3. Rabbit injected toxin. . . . 



4. Rabbit injected toxin 



5. Normal horse 



6. Normal rabbit 



7. Normal horse 



8. Control, no serum 



Control 

 anthrax 

 antigen 



+ 

 + + 



+ + 



TABLE 2 

 Second test of first group, following fresh bleeding two days later 



