THE NATURE OF TOXIN 



119 



The antigen in the form of the Na salts with cholesterol was 

 then tested for complement fixing power with the serums of 

 rabbits immunized with washed Megatherium organisms, and 

 with Megatherium hemotoxin. The procedure in this experi- 

 ment was identical with that used in testing the Diphtheria 

 antigen. The results appear in table 7. 



TABLE 7 



+ + = no hemolysis, complete inhibition; 

 complete hemolysis, no inhibition. 



+ = partial hemolysis; — = 



Table 8 shows the results of the agglutination-precipitation 

 tests of the same serums with the artificial fat antigen. 



TABLE 8 



Each tube contained: Antigen solution 0.08 cc, serum 0.2 cc. and salt solution 

 1 cc. The tubes after mixing and shaking were placed in the ice box over 

 night and read the following morning. 



+ = precipitation; — = cloudy, no precipitation. 



JOURNAL OF BACTERIOLOGY, VOL. VI, NO. 1 



