THE NATURE OF TOXIN 



121 



TABLE 10 



1. Rabbit injected, salt solution emulsion 



2. Rabbit injected, salt solution emulsion 



3. Rabbit injected, broth emulsion 



4. Rabbit injected, broth emulsion 



5. Normal rabbit 



6. Normal rabbit 



Control, no serum 



ANTIGENS 



Megatherium 

 suspension in 

 salt solution 



+ + 



+ 

 + 

 ± 



Artificial 

 antigen 



+ + 



+ 

 + 



TABLE 11 

 The same serum agglutinated a suspension of washed Megatherium in salt solution 



Each tube contained: Salt solution suspension 1 cc. and 0.04 cc. serum. Tem- 

 perature 4°C. for two hours. 



+ -| — h = complete agglutination and precipitation; ++ = almost complete 

 agglutination; 4- = partial agglutination; — = no agglutination. 



TABLE 12 

 Shmving the presence of antihemolysis in the same serums which were tested against 

 the clear centrifugated lysin of a twenty-four hour veal-Bacto peptone 1 per cent 

 broth culture of which 0.5 cc. caused complete hemolysis of 1 cc. of a 2 per cent 

 suspension of rabbit red cells in salt solution in eight minutes at 37°C. 



Each tube contained: 0.5 cc. of fresh hemolysin, 1 cc. of cell suspension and 0.04 

 cc. of serum. The tubes were shaken and placed in a water bath at 37°C. 

 for two hours, and then allowed to stand at 20°C. over night. 



— = no hemolysis; ++ = complete hemolysis. 



