STREPTOCOCCUS HEMOLYTICUS 



173 



Experiment V. The buffer action of horse serum in broth 



To investigate further the buffer effect of horse serum titra- 

 tion curves of broth containing 1 per cent glucose, 1 per cent 

 glucose plus 5 per cent horse serum, and 1 per cent glucose plus 



n 



hi 



osiKHTO^ 



Fig. 1. Experiment V 



10 per cent horse serum were plotted after the following proce- 

 dure had been carried out: To 10 cc. portions of the three types 

 of broth mentioned above, amounts of n/50 acetic acid varying 

 from 1 to 12 cc. were added and the Ph taken. The curves were 



