214 



LAURENCE F. FOSTER 



to remove the lactic acid, i\.bout 30 cc. of water were added to 

 the ether extract and the ether removed by distillation. The 

 water extract remaining was then titrated with n/10 Ba(0H)2 

 adding a 5 cc. excess of the base. The material was boiled for 

 fifteen minutes to convert all of the lactic acid to the barium 

 salt, after which the excess of Ba(0H)2 was removed by neutrali- 

 zation with H2SO4. This mixture was allowed .to stand for 

 several hours on a steam bath, filtered, and the filtrate and 



V30 



80 70 60 5410 3920 10 3 



Fig. 1 



washings evaporated to dryness. After taking up the residue 

 with water the material was again filtered to remove traces of 

 organic matter or carbonates. The filtrate was then made up 

 to a definite volume (50 or 100 cc.) and a 10 or 20 cc. aliquot 

 taken for analysis. A test for succinic acid was made at this 

 point by adding to the ahquot sufficient 95 per cent alcohol to 

 bring the volume to 100 cc. Succinic acid, if present, gives a 

 precipitate, and the material must be filtered. The filtrate was 

 again evaporated to drjniess and the residue taken up in 60 per 



