216 



LAURENCE F. FOSTER 



curve is not typical of a mixture of two volatile acids. No 

 attempt has been made to re-fractionate the distillates, conse- 

 quently the percentage of each acid in the volatile portion is not 

 known. Reference to table 3 indicates that no close agreement 

 exists between the per cents of volatile acid from culture (1) 

 and culture (2) though the same lot of broth was used in each 

 and the fermentation apparently progressed to the same point 

 as the final Ph of the cultures was practically equal. Culture 

 (3) gave a fraction of volatile acid still smaller than was noted 

 in the other cases. 



TABLE 2 

 Experiment I 



The lactic acid estimations in the two glucose broth cultures 

 do not correspond especially well and the value obtained in 

 culture (3) is smaller than would be expected. 



Through a lack of time it has been impossible to carry the 

 present phase of the investigation to a logical completion. As 

 a consequence it will be inadvisable to draw other than very 

 general conclusions from the data presented. 



It may be concluded from results that are to be presented 

 later that in a 1 per cent glucose broth culture of the strepto- 

 coccus some 156 mgm. of glucose are utilized in the first twelve 

 to eighteen hours. From the data shown in table 3 it is possible 



