BIOCHEMISTRY OF STREPTOCOCCUS HEMOLYTICUS 



223 



noted that although a rough paralleUsm between these factors 

 is shown in the three curves of each culture it is most striking 

 in the pleural fluid culture (fig. 3). Here, particularly in the 

 maximum period, may be seen a close relationship between the 

 curves of growth, acid formation, and glucose utilization. A 

 correspondence of the three factors in the maximum period 



TABLE 5 

 Experiment III 



BACTERIAL COUNT 



Per cu. mm. 



Log 



Ph 



Per 



100 cc. 



Utilized 



NHs, 



MGM. N 

 PER 

 100 CC. 



AMINO 



ACIDS, 



MGM. N 



PER 



100 CC. 



HEMOLYSIS 



(1) 1 per cent glucose broth (pleural fluid no. 198) 



(2) 1 per cent glucose, 5 per cent horse-serum broth (pleural fluid no. 198) 



(3) 1 per cent gucose 5 per cent horse-serum (Laboratory strain) 



appears in the other two cultures as well, though it is less striking. 

 In each case it will be observed that a rise in the acidity curve 

 is preceded by a rise in the growth curve. Attention should be 

 directed to the fact that while acid production proceeds most 

 rapidly during the time when the organisms are multiplying 

 at a maximum rate, nevertheless, a considerable lowering of 



