BIOCHEMISTRY OF STREPTOCOCCUS HEMOLYTICUS 



225 



6 and 7) was greatest between the third and the sixth hours 

 while in the glucose culture (fig. 5) it was largest between the 

 sixth and the ninth hours. The increase in anunonia output in 

 a general way parallels growth and acid formation during the 

 maximum period in each case. Associated with this increase 



Vh 



SZ 

 SjS 

 6.0 



C.4 



CB 



l.t 



7.6 



3 € 9 Houfs 



Fig. 3. Experiment III. Culture (2) ; 1 Per Cent Glucose, 5 Per Cent Horse 

 Serum Broth (Pleural Fluid Strain) 



in the output of ammonia a coincident decrease in amino acid 

 production is evident. In the serum cultures the curves of 

 amino acid output rise sharply at the sixth hour while in the 

 glucose culture the rise is delayed until the ninth hour. The 

 initiation of this rise appears to be in dhect correlation with a 

 high point of the ammonia curve. 



