BIOCHEMISTRY OF STREPTOCOCCUS HEMOLYTICUS 



229 



ammonia formation is the result of intracellular deaminization 

 of assimilated protein material. 



Wolf and Harris (1917b) in their study of the biochemistry of 

 Clostridium welchii and C. sporogenes noted in cultures grown in a 

 medium of high amino acid content that at the close of the experi- 

 ment the concentration of amino acids was less than at the begin- 



(nf^- per 10 Oct) 



/{tnino «.«'V N 

 (m^- 7>er 100 tt) 



ZO 



Hours 



Fig. 7. Experiment III. Culture (3) ; 1 Per Cent Glucose, 5 Feb Cent Horse 

 Sbrtjm Broth (Laboratory Strain) 



ning. This indicates that these substances were assimilated by 

 the growing organisms and destroyed through deaminization. § 

 Reference to figures 6 and 7 reveals a very decided difference 

 in nitrogen metabohsm in the two serum cultures during the 

 initial three-hour period. The pleural fluid culture exhibited a 

 definite decrease in amino acid output correlated with an increased 



